Rapid Detection of Tebuconazole Based on Aptasensor and Aggregation of Silver Nanoparticles

Tebuconazole is a triazole fungicide used in agriculture to treat pathogenic fungi. It listed as possible carcinogen and it shows potential risk for the environment at very low concentration. Therefore, detection monitoring of tebuconazole food play an important role. The current methods analysis employ gas-liquid chromatography (GLC) high-performance liquid (HPLC) after sample extraction with organic solvents column cleaning. Besides advantages these such efficiency, repeatability, accuracy, they are still time-consuming costly. Herein, we report simple, sensitive platform fast pesticides cost. technique exploits pesticide-specific DNA aptamer bioreceptor optical biosensor. Instead trying capture pesticide on sensor surface, our method allows aptamers, which adsorbed nanoparticle’s detach from nanoparticles when interacting pesticide. This leads pesticide-induced aggregation change absorption spectrum metallic upon high-salt concentrations, can be monitored unaided eye or absorbance measurement. Using model analyte pesticide, designed aptasensor showed high sensitivity selectivity limit ~10 nM reaction time within ~20 min. In case spiked rice samples, average recoveries were range 89.90–110.86% relative standard deviations (RSD) 3.11–4.32%. These results indicate that sensing exploited rapid real samples.